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The combined SRB was dissolved with 100 l 10 mM Tris base buffer (pH 10.5) and absorbance was measured at 495 nm with a microtiter plate reader (BioTek, USA)

Epidemiology of gallbladder disease: cholelithiasis and cancer

While liver biopsy remains the gold standard for fibrosis evaluation, its invasiveness and associated risks make it less suitable for routine clinical application [52]