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After the behavioral study, the experimental animals were first anesthetized with ketamine (80 mg/kg) and xylazine (5 mg/kg) given intraperitoneally to ensure deep anesthesia and lack of pain perception

doi: 10.1111/j.1751-1097.2008.00478.x 216 van HeyningenR

The structure of these protein-substrate complexes allowed delineating the residues contributing to both the G and H sites that form the active site cavity