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At Day 4, cells were trypsinized and collected for Fluorescence-activated cell sorting (FACS) to select GFP-expression cells in the middle of the expression distribution for generating polyclonal U2OS cell line

The ferroptosis catalyst reversed the reducing effect of H 2 O 2 on GSH levels to a certain extent

smegmatis mc 2 155 strain was grown as described above until the culture reached an OD 600 of 0.6

Bridging these gaps will require the integration of human-relevant models such as ovarian organoids and microphysiological systems, the development of validated biomarkers and potency assays, and the execution of large-scale, well-controlled clinical trials with long-term follow-up