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After gently washing twice with PBS to remove detached cells, the culture medium was replaced with serum-free DMEM

The current acceptable daily intake (ADI) is 3.7 mg/kg body mass/day for nitrates and 0.07 mg/kg body mass/day for nitrites [9,10]

Therapeutic strategies targeting mitochondrial dysfunction for comorbidity intervention (Table 2) Enhancing mitochondrial energy metabolism Mitochondrial protectants Coenzyme Q10, a well-established clinical therapeutic agent, is a component of the ETC and plays a dual role in facilitating electron transfer and exerting potent antioxidant effects