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Effects of long-term whole-body cold exposures on plasma concentrations of ACTH, beta-endorphin, cortisol, catecholamines and cytokines in healthy females

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Timing may influence the peptides effects on slow-wave sleep and overall sleep quality

Samples were removed from the water bath, 250 NRS was added, and each was placed into a rotisserie hybridization oven at 37C with rotation at high speed for the appropriate incubation time (10, 20, 30 40 or 60 minutes)