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Despite their advantages, sequencing methods have limitations, including amplification biases, challenges in species resolution and difficulty in understanding functional roles [88]

Together, the results show that our sensitive assay enabled establishment of NAD and glutathione values in a large healthy population cohort, serving as a basis to interpret normal and abnormal values in whole-blood samples

This mechanism underscores the intricate relationship between oxidative stress, inflammation, and BBB integrity, all of which are critical factors in the development and progression of neurodegenerative diseases like Alzheimers disease (AD)

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