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Once you puncture the vial, however, you've created a potential entry point for contaminants

PSC supernatants containing high concentrations of MCP-1 enhanced the migratory ability of BMDM, and the expression of MCP-1 and F4/80 was significantly reduced in the pancreas of baicalin-treated CP mice, indicating that baicalin could inhibit macrophage migration by reducing the level of MCP-1 in the supernatant of PSCs, which was caused by the inhibition of TGF--activated kinase 1, TGF-1, TGF-R1, NF-B, and other signaling pathways in PSCs (119) ( Table 2 )