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Lancet 397, 15771590

To visualize H 2 O 2 -driven PDI oxidation by GPx7 and the luminal domain of GPx8, reduced PDI (10 M) was reacted with GPx7 or GPx8 (1 M each) in the presence of 10, 50 or 200 M of H 2 O 2 , and the redox-state change of PDI was monitored by mal-PEG 2K modification of free cysteines followed by non-reducing SDS-PAGE (Fig

196 showed that cystine levels are lower in tumour interstitial fluid in murine pancreatic adenocarcinomas (PDACs) than in plasma, which may indicate that cancer cells actively uptake cysteine