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LC-MS/MS Analysis With DDA Samples were injected (30 L) onto an Aeris PEPTIDE XB-C18 100 2.1 mm column, with solid core 1.7 m particles (100 ) fitted with a SecurityGuard ULTRA C18-peptide guard column (Phenomenex, Torrance, CA) using a Nexera UHPLC system (Shimadzu, Columbia, MD) with water (A) and ACN (B), both containing 0.1% formic acid, at a flow rate of 0.3 mL/min (40C)

Depletion of GSH with the GSH oxidant diamin disrupted the meiotic spindle apparatus ( 2 O 2 concentrations during in vitro maturation displayed concentration dependent increased incidences of misaligned chromosomes, spindle abnormalities, and aneuploidy ( A number of studies have shown the ability of antioxidant treatments to protect mouse oocytes from chromosome and spindle misalignments and aneuploidy

29 a illustrates the inhibitory response that was observed with daily intraperitoneal injections at two doses (10 g/kg/day and 100 g/kg/day) of [D-Nle-Cys-Ile-NH(CH 2 ) 5 CONH 2 ]

Also, the absorbance is changed indicating a reaction (peaks) between iron and the chelating agent