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sgRNA complexes are synthesized by mixing equal volumes of crRNA (2 L, 160 M in nuclease free water) and tracrRNA (2 L, 160 M) and are incubated at 37 C for 30 min before Cas9 (2.7 L, 10 g/L, Alt-R S.p

Matthay MA, Zemans RL, Zimmerman GA, et al

Schwermetalle gehren dabei zu den wichtigsten zellulren Stressfaktoren, die nicht nur oxidativen Stress erzeugen, sondern auch die fr das berleben der Zellen wichtigen antioxidativen Schutzmechanismen hemmen, bzw