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After washing with PBS, the nuclei were counterstained with Hoechst 33342 (1:1000), and coverslips were mounted with Dako fluorescent mounting medium (S302380-2, Agilent Technologies)

According to Kaplan-Meier plots, patients with elevated levels of MMA had significantly increased cumulative incidence of cardiovascular and heart-specific death (Supplementary Fig

Apoptosis is induced via two main routes involving either the mitochondria (the intrinsic pathway) or the activation of death receptors (the extrinsic pathway)

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