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Baizabal Carvallo, J

The percent (%) cytotoxicity was determined using the following formula for each given experimental treatment: Cytotoxicity (%) = 100 [(Experimental Culture Medium Background)/(Maximum LDH Release Culture Medium Background)]

Gel permeation chromatography A Superdex 200 HR 10/30 column run on an kta purifier system (GE Healthcare GmbH, Freiburg, Germany) was equilibrated under anaerobic condition in an anaerobic chamber with protein buffer I and calibrated using the Gel Filtration Molecular Weight Markers Kit MWGF200 (Sigma Aldrich, Chemie, Taufkirchen, Germany) composed of cytochrome c ( M r = 12,400), carbonic anhydrase ( M r = 29,000), bovine serum albumin ( M r = 66,000), alcohol dehydrogenase ( M r = 150,000), -amylase ( M r = 200,000), and apoferritin ( M r = 400,000) according to the manufacturers instructions

For better understanding, several studies have focused on the anticholinesterase role of Piper nigrum extracts and constituents in animals