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Description

Acta Pharmacol Toxicol, 52: 95-99

These cells were pre-incubated for 48 h in low glucose medium before seeding for the migration scratch assay

For specific staining of the dead cells after expression of WD-associated G53S or M128V PrP variand, control, and treated cells were washed in warm media and incubated for 1 h at 37 C in the incubator with BOBO3 (Life Technologies) diluted 1:1000 in the media