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Chromatographic separation of AAs was achieved by applying 3 l of dissolved sample on a SeQuant ZIC-HILIC column (3.5 m particles, 100 2.1 mm) (Merck), combined with a Javelin particle filter (Thermo Fisher Scientific), and a SeQuant ZIC-HILIC precolumn (5 m particles, 20 2 mm) (Merck) using a linear gradient of mobile phase A (5 mM NH 4 OAc in acetonitrile/H 2 O (5/95, vol/vol)) and mobile phase B (5 mM NH4OAc in acetonitrile/H 2 O (95/5, vol/vol))

Thymosin Alpha-1 (abbreviated to T1) is a peptide hormone that occurs naturally in the body

EXPECTED EFFECTS BOOK NOW 12 Weeks: Noticeable reduction in inflammation and pain

Figure 2C)