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A 0.20 mL of dilute preparation was added to 2.90 mL of color reagent, the mixture incubated at 37 for 10 min and the absorbance was read at 412 nm on spectrophotometer against reagent blank

In the nucleus, Nrf2 promotes glutathione synthesis and regeneration by binding to antioxidant response elements on DNA and subsequently activating the transcription of antioxidant enzymes

However, the authors were of the view that the accumulated antioxidant may be a primary carotenoid called lutein

Basak U, Sarkar T, Mukherjee S, Chakraborty S, Dutta A, Dutta S, Nayak D, Kaushik S, Das T, Sa G (2023) Tumor-associated macrophages : an effective player of the tumor microenvironment