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We, thus, used Grx1-roGFP2 as a tool to monitor the GSH/GSSG redox state of E

After 4 days, the TS2/16 scFv-Fc was captured from the conditioned media with CaptivA HF protein-A affinity resin (Repligen, CA-PRI-0100) and eluted with IgG elution buffer (Thermo Scientific) containing 300 mM NaCl and neutralized with 100 mM Tris pH 8.0 to reach eventually pH 7.0