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Mitochondrion-targeting drugs can induce tumor mtDNA oxidation and release to the cytoplasm, activate the cGAS-STING pathway, while mitochondrion Lipid peroxidation and ROS can also promote mtDNA release

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The second stage is completed by glutathione synthetase (GS), whose substrate -Glu-Cys undergoes peptide bond condensation with glycine, accompanied by the hydrolysis of ATP to provide the required reaction energy