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Next, proteins were transferred to a 0.2 M PVDF membrane (Bio-Rad) for 16 h at 30 V in transfer buffer (25 mM Tris base, 192 mM glycine, 20% vol./vol

Ascites fractionation For ascitic fluid fractionation by molecular weight, ascitic fluids were diluted twice with PBS and then centrifuged sequentially through 100, 30, 10, and 3 kDa molecular weight cut-off spin concentrators (Sartorius, #Z614297, #Z614246, #Z614211, #Z629405)

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The moderately acidic environment can trigger Fe 4 Se 2 W 18 NCs to release Fe 3+ , which can be reduced by GSH to produce Fe 2+ along with the decomposition of H 2 O 2 for OH production, rendering cancer cells more susceptible to oxidative stress (Zhou et al., 2021)