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Methods Cell lines Human HPV-associated HNSCC SCC090 (RRID: CVCL_1899), SCC152 (RRID: CVCL_C058), SCC 154 (RRID: CVCL_2230), and UDSCC2 (RRID: CVCL_E325) and HPV-independent cell lines UMSCC22A (RRID: CVCL_7731), HN30 (RRID: CVCL_5525), HN31 (RRID: CVCL_5526) and UMSCC47 (RRID:CVCL 7759) were maintained in high glucose DMEM, MEM or RPMI as previously described [20, 21], supplemented with 10% fetal bovine serum, 1% glutamine, 1% vitamins, 1% non-essential amino acids, 1% penicillin/streptomycin, 1% sodium pyruvate, and 0.2% MycoZap Prophylactic (LT07-318, Lonza) to prevent mycoplasma overgrowth

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So, understanding right off the bat that smoking is bad for oral health is the first reason you cant smoke after tooth extraction