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With earnest eyes, and round, unthinking face, He first the snuff-box open'd, then the case." The mode of "tapping the box" before opening was characteristic of the beaux and fops of this period, and is commented on in a poem on snuff: "The lawyer so grave, when he opens his case, In obscurity finds it is hid, Till the bright glass of knowledge illumines his face, As he gives the three taps on the lid." Spain, Portugal, and France early in the Seventeenth Century became noted as the producers of the finest kinds of snuff

HPLC and LC-MS/MS analyses The 5-mC content in the sperm genome was quantified by HPLC as described with slight modifications 64

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