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Description

HDR homology directed repair editing of cells may be performed to insert or knock-in an exogenous gene, such as an exogenous gene encoding a CAR or a TCR, at a preselected locus in a cell

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An example illustration of such a hairpin structure is provided in the lower portion ofFigure 13B, where the portion of thesequence5' of the final "N" and upstream of the loop corresponds to the tracr mate sequence, and the portion of the sequence 3' of the loop corresponds to the tracr sequence

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