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After incubation, all lysates were mixed with an equal volume of 2 urea sample buffer, heated at 65C for 5 minutes, and analyzed by Western blot

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Blood samples (300 L for rat and 1 mL for dog) were drawn at 0.083 (dog only), 0.25 (rat only), 0.5 (dog only) 1, 3, 7, and 24 h post-dose into K2 EDTA-containing tubes