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The mobile phase consisted of a linear gradient system of phase A (60% acetonitrile/40% water solution containing 10 mM ammonium acetate and 0.1% formic acid) and phase B (isopropanol:acetonitrile, 9:1, v/v): 0 min 60% A, 2 min 57% A, 12 min 40% A, 12.1 min 25% A, 18 min 1% A, 19 min 1% A, and 20 min 40% A

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Revised Date: January 18, 2023 Recommendations and Topics Scope Key Recommendations Background Epidemiology Patient Risk Factors Associated with B 12 Deficiency Patient Risk Factors Associated with Folate Deficiency Prevention Diagnosis Management Ongoing Care References Resources Appendix A: Dietary sources of cobalamin Scope This guideline covers the primary care investigation and management of cobalamin (vitamin B 12 or simply B 12 ) and folate deficiency in adults