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The cells were incubated at room temperature for 15 minutes, and 400 l of 1x binding buffer was added and analyzed by flow cytometry immediately

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Sonodynamic therapy is a noninvasive antitumor approach that generates ROS through the simultaneous interaction of acoustic sensitizers, molecular oxygen and low-intensity ultrasound waves, leading to apoptosis and necrosis of cancer cells while minimizing damage to adjacent normal tissues [195]