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Sample lysates were separated via 414% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), following which the proteins were transferred to polyvinylidene difluoride (PVDF) membranes

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The functions of amylin are complementary to insulin and to GLP-1 : Slows gastric emptying , similarly to GLP-1, but more strongly Suppresses glucagon , helps control postprandial glycemia Induces satiety , via the area postrema in the brainstem Regulates weight balance , long-term effect In type 2 diabetes amylin secretion deteriorates in parallel with insulin secretion , as the disease progresses, the -cells lose the ability to produce both hormones at once