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PMID: 300 XiaoH-HZhuY-XLuLZhouL-PPoonC-WChanC-Oet al

The protein content of RCM was determined using a BCA assay kit

Quality filtered 16S rRNA sequences were clustered into operational taxonomic units (OTUs), with 97% similarity, by closed reference OTU-picking using the UCLUST algorithm and GreenGenes reference database (v13.5) as implemented in Quantitative Insights into Microbial Ecology (QIIME versions 1.6 and 1.7) [64]