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Toxicol ., 26 (9), 697703

Development of a lauric acid/albumin hybrid iron oxide nanoparticle system with improved biocompatibility

However, alternative routes for cytosolic NADPH production exist, including isocitrate dehydrogenase 1 (IDH1), malic enzyme 1 (ME1), methylenetetrahydrofolate dehydrogenase and formyltetrahydrofolate dehydrogenase, and can often compensate to generate the required NADPH for biosynthesis

The membranes were blocked with 5% nonfat milk in Tris-buffered saline at room temperature for 1 h and then probed with primary antibodies overnight at 4C, followed by HRP-conjugated secondary antibodies at room temperature for 1 h