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CS activity was measured by spectrometric quantitation (412 nm) of 5,5dithiobis-2-nitrobenzoic acid conversion to 2-nitro-5-thiobenzoic acid in the presence of coenzyme A thiol generated during citrate production (CS0720, Sigma) as previously described [20]

In the general population, lower levels of albumin are associated with increased overall mortality.4145 The reverse is also true, as in the normal albumin range, albumin levels higher than 43 g/L are associated with a 20%40% decreased risk for all-cause mortality.46 Albumin contributes to 75% of the plasmatic oncotic pressure, which is disproportionally high since albumin only accounts for 50% of plasma proteins.28 That can be explained by albumins low molecular weight, which results in high osmotic activity per gram.23 Additionally, the osmotic activity of albumin increases by its negative charge, which holds osmotically active sodium cations (Gibbs-Donnan effect).47 Albumin has other important functions namely its binding capacity, anti-oxidant and anti-inflammatory properties.15,16 It has three essential functional domains: A metal binding domain

Therefore, the effectiveness of CYANOKIT for cyanide poisoning was established based on the results of the adequate and well-controlled animal efficacy study described below

Olney JW, Farber N