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LC-MS/MS measurements LC-MS/MS analysis was performed for each sample using a nanoLC EASY 1200 system (LC-030378, Thermo Scientific, USA) coupled to a Q Exactive Plus mass spectrometer (03893 L, Thermo Scientific, USA) at the Mass Spectrometry Unit of CENTD (Instituto Butantan, Brazil)

In addition, studies have explored its association with molecular signals linked to tissue remodeling, suggesting a supportive role in recovery mechanisms (16)

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For research the pure form is more flexible, it allows testing of stand-alone Cagrilintide as well as experimental combinations