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the starting DNA segments are reassembled (&/or subjected to one or more directed evolution methods described herein) to generate a library of recombinant DNA segments varying in size which will include full length coding sequences and any essential regulatory the starting DNA segments are reassembled (&/or subjected to one or more directed evolution methods described herein) by any of the sequence reassembly (&/or one or more additional directed evolution methods described herein) formats provided herein to generate a diverse library of recombinant DNA segments