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Cells were then incubated with the monoclonal anti-tyrosine antibody (1:25 in PBS, 1% BSA for 30 min at 37C, washed (3 10 min and incubated with the mouse monoclonal anti-goat antibody labeled with Alexa Fluor 488 (Invitrogen, Karlsruhe, Germany, 1:100) for 30 min at 37C

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These conditions are strongly linked to metabolic dysregulation and energy equilibrium disruption, positioning them as central topics in metabolic research